Anaerobic organisms play critical roles in health and disease pathology of humans and animals, as well as throughout the Earth’s ecosystems. Isolating and culturing these fastidious organisms with consistent results requires specialized conditions.
Your research can benefit from our decades of experience with formulating and manufacturing special nutritive TruPRAS™ media that never comes into contact with oxygen. We provide a wide range of media for specimen collection and transport, enriched plated agar media, and enriched liquid media specifically designed for culturing even organisms that were previously thought to be unculturable.
The Oxygen-Free Difference
From pouring to packaging, the absence of oxygen in TruPRAS media prevents the formation of harmful byproducts. When opened in oxygen-free conditions, you can be sure that oxidative damage is ruled out as a variable. And since it can be stable at room temperature and is ready to use right out of the package, oxygen-free also means no wasted time.
TruPRAS media is pre-reduced, anaerobically sterilized media, and describes a medium whose oxygen has been removed before heat sterilization. The medium is autoclaved, dispensed, and packaged under oxygen-free conditions, which prevents the reaction of reducing agents with oxygen, which can lead to the formation of by-products that are harmful to, or can limit the growth of, anaerobes in that medium.
TruPRAS media, when packaged oxygen-free in foil packets, can be stored at room temperature. TruPRAS media also requires no pre-reduction steps by the end user and is ready for use as soon as the package is opened.
Our culture media can be opened and exposed to oxygen for up to 30 minutes if needed. We recommend placing the culture media inside an anaerobic environment such as an anaerobic chamber or jar, immediately after opening or inoculating the media. Oxygen can react with the culture media, forming by-products that are toxic to anaerobic organisms.
Of course, if you are planning to use TruPRAS media in an aerobic environment, with facultative or aerobic organisms, exposure to oxygen is completely fine and will not affect its performance.
Media Products:
- Agar Plates
- Agar Combo Packs
- Liquid Broth (Tubes & Bottles)
- Bagged Media
- Specimen Collection & Transport
- ID Discs & Reagents
- Microbiome & Research
- Clinical Isolation & Id
- Clostridium Difficile
- Custom Media - Biolog can manufacture plated and broth media in various formats such as standard petri dishes, square/rectangular dishes, large volume bottles, bags, and more. We will produce your culture media using our TruPRAS™ manufacturing process as well as perform strict quality control on every batch.
Looking to acquire a new anaerobic chamber? Check out our models AS-150 & AS-500.
Product Literature: MKT-AS-102-Rev-B-TruPRAS-Brochure.pdf