MitoPlates from Biolog provide a powerful new research tool by allowing scientists to run preconfigured sets of 96 mitochondrial function assays in one experiment designed to measure the effects of a diverse set of substrates and inhibitors on mitochondrial function. Mitochondria can be interrogated and characterized in novel ways, looking at rates of substrate metabolism, sensitivity to drugs and other chemicals, and effects of mutations in mitochondria-related genes.
Investigate how mitochondria change with:
• Cell differentiation
• Cancer & ageing
• Neurological disorders
• Metabolic disorders
• Immune cell activation
• Bacterial/viral infection
• Inborn genetic defects
MitoPlates™ are cell-based phenotyping assays used to interrogate and characterize mitochondria. Any cell type of interest can be added to the plates along with Biolog’s proprietary redox dye. Mitochondrial function is assayed by measuring the rates of electron flow into and through the electron transport chain from metabolic substrates that produce NAD(P)H or FADH2 such as L-malate, succinate, pyruvate, etc. Each substrate follows a different route, using different transporters to enter the mitochondria and different dehydrogenases to produce NAD(P)H or FADH2. The electrons travel from the beginning (complex 1 or 2) to the distal portion of the electron transport chain where a tetrazolium redox dye (MC) acts as a terminal electron acceptor that turns purple upon reduction. Additional MitoPlate assays probe the sensitivity of the mitochondria to a set of 22 diverse inhibitors.
When using Phenotype MicroArrays, you need an instrument that will read at the right wavelengths and measure the changes kinetically over time at short intervals. You also need a software package to help you analyze all that data and identify the differences between your samples. With the Odin™ platform you can characterize phenotypes by monitoring growth curves and cell metabolism kinetics for microbial or mammalian cells for up to 50 plates at the same time. When combined with Phenotype MicroArrays, Odin can analyze up to 4,800 conditions unattended for hours or days.
- Odin™ family: Fully automated incubator/readers with up to an 8-plate capacity (Odin VIII) or a 50-plate capacity (Odin L) with software that can identify aerobic and anerobic bacteria, yeast, filamentous fungi and allows you to do phenotypic analysis.
Product Literature: MitoPlate Instructions For Use, Br_Mitoplate Flyer, Br_MitoPlate White Paper, MitoPlate SDS
Visit Biolog's website for more information: MitoPlate Phenotype