The Anaerobic MediaMatcher™ (AN-MM) microplate addresses the difficulties of culturing complex anaerobic organisms, especially those in the gut microbiome, and is also useful for applications like anaerobic fermentation and prebiotics.
Culturing obligate anaerobes can be labor-intensive and costly due to media optimization. The Anaerobic MediaMatcher microplate simplifies this with a diverse selection of media options for sampling, streamlining your workflow and enhancing research efficiency.
Key Features
- Simplified Media Selection - pre-loaded with 47 distinct media formulations in duplicate, making it easy to select the optimal one.
- Optimized Experimental Design - Design of Experiment (DOE) principles used to cover a wide range of variables that support growth of anaerobes.
- Efficient and Cost-Effective - low-volume configuration allows for screening multiple strains at once, with less waste than traditional methods.
- Enhanced Data Analysis - works with the Odin family of instruments and software to provide insights into kinetic growth profiles and support media selection.
- Compatible with Single Isolates or Communities - supports co-culture of multiple organisms.
- Streamlined Anaerobic Research - integrates seamlessly with PreBioM plates for an all-inclusive solution for phenotypic profiling of anaerobes.
- Scale up for Multiomics - 42 exclusive Biolog Synthetic Media recipes and 5 popular commercial medias available in larger volumes for other downstream methods.
Workflow
1. A challenging anaerobic microbe, isolated from a bio specimen, is subcultured on a rich agar and plated on an AN-MM plate within an anaerobic chamber.
2. The plate is sealed and transferred to Odin for 24 hours of incubation and analysis.
3. Kinetic OD measurements are employed to identify the optimal media (BSM7), which meets the researcher’s needs by promoting anaerobic growth while minimizing the lag phase.
4. BSM7 is acquired to replace the standard inoculating fluid specified for PreBioM plates, enabling metabolic phenotyping of this microbe.
Once you have identified the best media for your strains or community using Anaerobic MediaMatcher, you can move on and scale up. Purchase bottles of any Biolog Synthetic Media for downstream work.
Solution Offerings
| Name | Description |
|---|---|
| Anaerobic MediaMatcher | A microplate with 47 different media formulations in duplicate for optimizing microbe cultivation |
| Biolog Synthetic Media 1-42 | 500 mL bottles of BSM 1-42, for streamlining downstream work |
| Odin system | The all-in-one solution for cellular metabolic characterization, growth kinetics, and identification |
Product Literature: Anaerobic MediaMatcher & Biolog Synthetic Media Brochure
Visit Biolog's website for more information: Biolog Synthetic Media, Anaerobic MediaMatcher
Check out our Microbial ID and Phenotypic Characterization systems: Odin L and Odin VIII.