The RhizoPlate™ series of microplates provides a targeted and easy-to-use solution for detecting, ranking, and monitoring nitrogen-fixing & phosphate-solubilizing bacteria (NFB/PSB) within the rhizosphere.
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Advancing Sustainable Agriculture: Simplifying Biostimulant product development to reduce use of synthetic fertilizers.
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Comprehensive Analysis: Select for and evaluate NFB/PSB capability from bacterial isolates, microbial communities, and complex soil samples. Measure growth under a range of conditions at the same time to identify and evaluate the most robust strains or communities.
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Streamlined Relative Quantification: Kinetic optical density (OD) measurements based on the resultant growth from N2 or inorganic phosphate utilization are used to rapidly assess and compare the performance of NFB/PSB, which is ideal for strain screening.
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Persistence Monitoring: Track NFB/PSB in soil over time to guide cost-effective biostimulant dosing in the field.
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Scalable Throughput: Seamlessly integrates with the Odin platform for small-scale (Odin VIII) or higher-throughput (Odin L) screening operations, with powerful software for actionable insights.
Turnkey Solution:
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RhizoPlate N includes a 96-well plate pre-loaded with 30 carbon substrates in triplicate, specifically selected to support the unique growth requirements of NFB and a nitrogen-free inoculating fluid.
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RhizoPlate P provides a 96-well microplate uniformly coated with insoluble inorganic phosphate, and pre-loaded with 30 carbon substrates in triplicate, selected to support the unique growth requirements of PSB and a phosphate-free inoculating fluid.
Applications:
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Screening and Selecting Isolates:
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Rapidly screen through large strain collections
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Rank candidates
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Compare nitrogen fixation or phosphate solubilizing performance
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Select engineered strains to move forward for additional analysis
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Communities:
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Analyze complex soil microbial communities
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Develop biostimulants that combine complementary species or strains for broader substrate utilization
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Evaluate and compare the performance of multiple microbial communities on a single, integrated platform
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Monitoring Persistence in Soil:
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Monitor the presence of Nitrogen Fixing Bacteria and Phosphate Solubilizing Bacteria in agricultural fields
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Track PSB/NFB persistence over time
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Ensure optimal biostimulant dosing
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Maintain cost-effectiveness in application of new biostimulant products
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Product Literature: RhizoPlates Flyer, RhizoPlate MicroPlates, Application Note
Visit Biolog's website for more information: RhizoPlates for Sustainable Agriculture